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The influence of genetic and cardiovascular riskfactors on the CADASIL phenotype
Sumeet Singhal,1 Steve Bevan,1 Tom Barrick,1 Philip Rich2 and Hugh S. Markus1
1Clinical Neuroscience, St George’s Hospital Medical
Correspondence to: Professor Hugh Markus, Clinical
School and 2Neuroradiology, Atkinson Morley
Neuroscience, St George’s Hospital Medical School,
Neuroscience Centre, St George’s Hospital, London, UK
Cranmer Terrace, London SW17 0RE, UKE-mail: email@example.com
SummaryThe clinical phenotype in cerebral autosomal dominant
dependency or MRI lesion load. There was no evidence
arteriopathy with subcortical infarcts and leucoencephalo-
of intrafamilial clustering of particular phenotypes.
pathy (CADASIL), an autosomal dominant cerebral
Amongst subjects with stroke/transient ischaemic attack,
arteriopathy, is variable, but the reasons for this remain
smoking at the time of the event was independently asso-
uncertain. Possible factors include the mutation site and the
ciated with earlier age of onset (P = 0.01). There were no
influence of additional modulating factors, which could
associations between age of onset or presence of stroke and
include both epistatic interactions and interactions with
other cardiovascular risk factors, including homocysteine.
cardiovascular risk factors known to cause sporadic
Homocysteine levels were higher in migraineurs [mean
small vessel disease. In a large prospectively recruited
(SD) 12.8 (5.6) versus 9.8 (3.4) mmol/l, P = 0.02)] and elev-
cohort of CADASIL subjects we determined relationships
ated homocysteine was independently associated with an
between phenotype and mutation site, the apoE genotype
earlier age of onset of migraine (P = 0.01). No relationship
and cardiovascular risk factors. In addition to clinical fea-
was found between MRI lesion volume and risk factors, or
tures, disease severity was assessed by MRI lesion volume,
between apoE genotype and phenotype. Our results show
measured both semiquantitatively (Scheltens scale) and
no notch 3 genotype–phenotype correlations. This implies
quantitatively. One hundred and twenty-seven CADASIL
that modulating factors influence phenotype. Smoking
cases from 65 families with 17 different mutations were
appears to increase the risk of stroke, while high homocys-
studied. Site of mutation was not associated with the
teine levels are associated with an increased risk of
presence or age of onset of stroke, migraine, dementia,
Keywords: CADASIL; genotype–phenotype; homocysteine; risk factors; apolipoprotein E
Abbreviations: apoE = apolipoprotein-E; CADASIL = cerebral autosomal dominant arteriopathy with subcortical infarctsand leucoencephalopathy; EGF = epidermal growth factor; MMSE = Mini-Mental State Examination; OR = odds ratio;PCR = polymerase chain reaction; TIA = transient ischaemic attack
Received February 17, 2004. Revised April 28, 2004. Accepted April 28, 2004. Advanced Access publication June 30, 2004
IntroductionCerebral autosomal dominant arteriopathy with subcortical
cysteine residues. A few splice site mutations (Joutel et al.,
infarcts and leucoencephalopathy (CADASIL) is an autosomal
2000) and small in-frame deletions (Dichgans et al., 2000)
dominant cerebral arteriopathy caused by mutations in the
have also been reported, but these also result in an unpaired
notch 3 gene (Joutel et al., 1996). Characteristic features
number of cysteine residues within the EGF-like domain.
include migraine, recurrent subcortical strokes and subcortical
Despite the highly stereotyped nature of the mutations caus-
dementia. Mutations are highly stereotyped and result in the
ing CADASIL, marked phenotypic variation has been reported
gain or loss of cysteine residues in epidermal growth factor
(Chabriat et al., 1995; Dichgans et al., 1998). Some individuals
(EGF)-like domains in the extracellular portion of the trans-
present with stroke in their 20s, while others remain stroke free
membrane notch 3 protein. Each EGF-like domain contains
into their 60s. The age of onset of the subcortical dementia that
three paired cysteine–cysteine bonds. Almost all mutations are
frequently develops is variable. Migraine is a common mani-
simple missense mutations resulting in an unpaired number of
festation, but many patients remain migraine-free throughout
Brain Vol. 127 No. 9 # Guarantors of Brain 2004; all rights reserved
life. A number of other neurological presentations, including
disease progression, particularly the extent of leucoaraiosis,
epilepsy and an acute reversible encephalopathy (Schon et al.,
could be the apolipoprotein-E (apoE) genotype. Presence of
2003), have been reported, but these affect a minority and early
the «4 allele predisposes towards Alzheimer’s disease
reports suggest they do not seem to segregate within families or
(Strittmatter et al., 1993) and has been reported to influence
with particular mutations. With the exception of a single report
the extent of cerebral damage following a number of
suggesting that a novel C455R mutation located in the pre-
different brain injuries, including traumatic brain injury and
dicted ligand-binding domain may be associated with an earlier
subarachnoid haemorrhage (Niskakangas et al., 2001), and
age of stroke onset (Arboleda-Velasquez et al., 2002), previous
in patients with cerebral amyloid angiopathy (Greenberg
studies suggest the site of the notch 3 mutation has little bearing
on phenotype. However, there have been limited systematic
In this study we examined factors affecting the CADASIL
studies examining genotype–phenotype correlations. The lack
phenotype in a large cohort of CADASIL individuals recruited
of clear genotype–phenotype correlations has led to the sug-
from Great Britain. We determined whether the site of the
gestion that other factors may modulate the disease process.
notch 3 mutation itself, conventional cardiovascular risk fac-
These could include both environmental and other genetic
tors, homocysteine and the apoE genotype influenced pheno-
factors, but the role of either has not yet been explored.
type. We sought to detect influences both on the extent of
CADASIL is characterized by a systemic arteriopathy,
visible diffuse cerebral injury, represented by the degree of
which specifically affects the cerebral small vessels. Damage
leucoaraiosis, and on acute ischaemic injury, presenting as a
is particularly severe in smooth muscle cells in the media,
stroke or transient ischaemic attack (TIA). We therefore exam-
although endothelial changes have also been reported. The
ined the relationship between putative risk factors and both
most attractive disease hypothesis is that these diffuse changes
MRI measures of total leucoaraiosis lesion load, with the
result in reduced cerebral perfusion and inability of the cerebral
presence and age of onset of stroke/TIA, as well as other
vessels to autoregulate. This is supported by imaging studies
showing both reduced resting perfusion and impaired vaso-
dilatory reserve in response to carbon dioxide (Pfefferkorn
et al., 2001) or acetazolamide (Chabriat et al., 2000).
The disease process can result in both acute focal lacunar
infarction and more diffuse ischaemic changes, referred to
One hundred and twenty-seven CADASIL cases from 65 familieswere studied. Individuals were recruited prospectively as part of a
radiologically as leucoaraiosis, and best seen as high signal
British CADASIL prevalence study, and all gave informed consent.
on T2-weighted MRI. Lacunar stroke is thought to arise from
The study was approved by the South Thames Multi Region Ethics
an abrupt disruption of perfusion and/or thrombosis in the
Committee. Diagnosis was confirmed by direct sequencing of the
perforating end arteries. It could be hypothesized, therefore,
notch 3 gene (123 cases), by skin biopsies showing the presence of
that any factor either exacerbating the diffuse arteriopathy or
granular osmiophilic material in combination with typical neuroima-
resulting in an increased tendency to thrombosis might be
ging appearances (two cases), and by typical neuroimaging appear-
associated with an earlier onset of stroke in patients with
ances in a family with biopsy-proven CADASIL (two cases).
The following phenotypic features were recorded: presence and
includes ischaemic demyelination, neuronal loss and gliosis
age of onset of stroke/TIA; presence and age of onset of migraine; the
(Ruchoux and Maurage 1997). It affects the white matter
Mini-Mental State Examination (MMSE) score (Folstein et al., 1975);
regions supplied by the same perforating vessels affected in
the presence of dementia, defined either as a previous diagnosis ofdementia made by a neurologist/psychiatrist, or an MMSE score <23,
lacunar stroke. The neuroimaging changes in leucoaraiosis are
which has been found to be consistent with DSM-III-R (Diagnostic
first seen in regions most distal to the origin of these perforating
and Statistical Manual of Mental Disorders) criteria for dementia
vessels, i.e. those in which perfusion pressure is lowest. Thus, it
(Zaudig, 1992); and a history of acute reversible encephalopathy or
is possible that factors resulting either in exacerbation of the
primary epilepsy. Functional dependence was measured using the
underlying arteriopathy or in increased susceptibility to
modified Rankin scale, a six-point scoring system which has been
neuronal or other brain tissue injury could increase this
validated in the assessment of handicap in stroke patients (van Swieten
One explanation for the marked variation in phenotype
Using a standard questionnaire and examination, the following
could be that vascular risk factors exacerbate the disease pro-
cardiovascular risk factors were assessed: hypertension, hypercholes-
cess and thus modulate the CADASIL phenotype. These could
terolaemia, smoking status, and diabetes mellitus. Hypertension was
include both conventional risk factors, such as hypertension
defined as an age-adjusted systolic blood pressure >140 mmHg, ordiastolic blood pressure >90 mmHg, or the prescribed use of anti-
and diabetes, which are recognized risk factors for non-
hypertensive medication. Hypercholesterolaemia was defined as an
inherited small vessel disease, and novel risk factors, such
age adjusted non-fasting cholesterol >6 mmol/l or the presence of
as homocysteine. Elevated homocysteine was reported in a
prescribed statin therapy. Smoking was studied in two ways: ever-
small study in patients with CADASIL (Flemming et al.,
smokers at the time of recruitment; and current smokers at the first
2001), and has been shown to be a risk factor for cerebral
ischaemic event. In a subgroup of 68 individuals, non-fasting serum
small vessel disease, possibly acting via endothelial dysfunc-
homocysteine was measured and hyperhomocysteinaemia defined
tion (Hassan et al., 2004). An alternative factor influencing
results. The logarithm of homocysteine level, the reciprocal of cho-
In 112 individuals in whom original MRI scans were available, lesion
lesterol level and the square root of MRI lesion load were taken to
load was assessed both by the semiquantitative Scheltens scale, and by
normalize distributions. For statistical analysis of the relationship of
quantitative measurement of lesion volume. The Scheltens scale was
phenotype with mutation site, the position of the mutated amino acid in
designed for measuring the load and spatial distribution of subcortical
the extracellular portion of the notch 3 receptor was determined. The
lesions in patients with sporadic vascular white matter disease, and
reciprocal of this was used to obtain a normal distribution. We also
has been shown to have good intra- and inter-observer reliabilities
compared lesion volume and clinical features between patients with
(Scheltens et al., 1993). Predefined regions are scored depending first
mutations in the predicted ligand binding domain and patients with
on size, and then on number of lesions. It was extended to include
mutations outside this domain. Patients were categorized as function-
scores for the anterior temporal lobe, external capsule and corpus
ally independent or dependent if they had Rankin scores of 0–2 or 3–5
callosum, as these regions have all been reported to be frequently
respectively. Univariate logistic regression was used to assess correla-
affected in CADASIL (Coulthard et al., 2000; Auer et al., 2001;
tions between risk factors and categorical outcomes (e.g. presence of
O’Sullivan et al., 2001). All scans were scored on the Scheltens
stroke/TIA). Linear regression was used for correlations with continu-
scale by the same consultant neuroradiologist (P.R.).
ous dependent variables (MRI scores). Multivariate analyses covary-
To derive a quantitative measure of lesion load, hard copies of
ing for gender and vascular risk factors, in addition to age, were carried
T2-weighted MRI scans were digitized and converted to a format
out when a statistically significant association was found on univariate
suitable for analysis using in-house software (# T. R. Barrick, St
analysis. To determine the effect of risk factors on the age of onset of
George’s Hospital Medical School, London). White matter lesions,
clinical outcomes, Kaplan–Meier analysis was used, followed by Cox
the brain circumference and the lateral ventricles were contoured
regression to determine the effect of controlling for other potentially
using commercially available image analysis software Dispunc
(# D. L. Plummer, University College London). Brain parenchymal
area was calculated as the difference between brain circumferentialareas and lateral ventricle areas. Infratentorial structures were not
included in the analysis, because of incomplete acquisition onsome scans. The most inferior slice for analysis was designated as
Clinical characteristics and risk factor profile
the first slice to show bilateral anterior temporal lobes as distinct
Eight cases identified through family screening were asymp-
structures. To account for differences in brain size between subjects,
tomatic. They had a mean age of 31 (range 20–45) years, and
total lesion load was expressed as a percentage of brain parenchyma.
four (50%) were female. The 119 symptomatic patients had a
All scans were analysed by the same person (S. S.). Intra-observer
mean age of 48 (range 21–82) years, and 72 (61%) were female.
reproducibility was determined on a random sample of 10 scans. The
At recruitment, stroke/TIA had occurred in 71 symptomatic
mean of the percentage difference in lesion volume between repeat
patients (60%), migraine in 92 (77%), dementia in 22 (19%),
lesion load estimation by the same observer was 8.74%. To determine
epilepsy in 13 (11%), and acute reversible encephalopathy in
within-subjects variation, the mean of the square of SDs of the twoscores for each scan was calculated (Bland, 2000). The within-
subjects SD, derived from the square root of this value, was 0.65%
Frequencies of cardiovascular risk factors were as follows:
[95% confidence interval (CI) À1.15% to þ 2.45%].
hypertension, 24/120 (20%); hypercholesterolaemia, 53/119(45%); current and ex-smoker, 67/126 (52%); diabetes
mellitus, 5/127 (4%); hyperhomocysteinaemia, 12/68 (9%).
Mean (SD) cholesterol (n = 115) was 5.6 (1.4) mmol/l, and
DNA was extracted from leucocytes using a commercial kit (Nucleon,Amersham Bioscience, Uppsala, Sweden), and polymerase chain
mean (SD) homocysteine (n = 68) was 12.2 (5.3) mmol/l.
reaction (PCR) amplification of notch 3 exons was performed. Primersused have been previously described (Markus et al., 2002). Notch 3mutations were sequenced directly with an ABI 377 automated
sequencer. Analysis of apoE subtypes was undertaken using pre-
viously published methods (Hixson and Vernier, 1990). Genomic
The distribution of notch 3 mutations is shown in Table 1. All
DNA was amplified by PCR in 10 ml of 75 mmol/l Tris–HCl (pH
were simple missense mutations resulting in the loss or gain of a
9), 0.01% (v/v) Tween, 1.5 mmol/l MgCl2, 0.25 U Taq polymerase
cysteine residue in one of the EGF-like repeats. After covary-
(Abgene, Epsom, UK), 20 mmol/l (NH4)2SO4, 0.1% (w/v) BSA, 0.2
ing for age, site of mutation was not associated with stroke/TIA
mmol/l each dNTP and each primer at 5 ng/ml. Thirty cycles of PCR
[odds ratio (OR) (95% CI) 1.118 (0.926–1.350), P = 0.25],
were performed at 94C for 30 s, 55C for 30 s and 72C for 1 min witha final extension step of 72C for 10 min. Products were then digested
migraine [OR 0.985 (0.817–1.188), P = 0.88], dementia
with 10 U of restriction endonuclease HhaI (NEB, Hitchin UK) at
[OR 0.782 (0.602–1.016), P = 0.07], acute encephalopathy
37C overnight and analysed by 2% low melting point agarose gel
[OR 0.938 (0.724–1.215), P = 0.63], primary epilepsy [OR
electrophoresis (Abgene). Genotype was determined by comparison
1.008 (0.721–1.409), P = 0.96] or Rankin dependency
of the digest pattern to known reference genotypes.
[OR 0.811 (0.623–1.057), P = 0.12]. There was no evidenceof intrafamilial clustering of any particular phenotype,
although power to detect this was limited by the small number
All dependent variables were analysed with age as a covariate. In
of affected individuals recruited in most families.
addition, continuous, normally distributed, independent variables
Site of mutation was not associated with quantitative MRI
were age-adjusted, and these values were used in all analyses and
lesion load [B regression coefficient (95%CI) À0.0003
(À0.075–0.074), P = 0.99] or with total Scheltens score
versus 6.56 (4.45) %, P = 0.093]; individual values are
[B regression coefficient À0.625 (À1.805–0.554), P = 0.38].
indicated in Fig. 1. Age-adjusted Scheltens score was also
Figure 1 shows the quantitative lesion load distribution plotted
slightly higher [61.81 (4.38) versus 53.55 (13.39), P = 0.058].
against age, stratified by affected exon.
Three cases, from a single family, had mutations (C440G) in
the predicted ligand-binding domain. The individuals were
Relationship between vascular risk factors and
aged 67, 56 and 37 years and presented with dementia at 66
years, migraine at 14 years and depression at 23 years; none had
experienced stroke or TIA. There was a non-significant trend to
Stroke/TIA. A history of stroke/TIA was positively associated
higher age-adjusted lesion volume in cases with this mutation
with age [OR 1.077 (1.040–1.116) per year, P = 0.0003]. After
than in cases with other mutations [mean (SD) 9.27 (SD 2.32)%
covarying for age, current smoking was associated with anincreased risk of stroke/TIA [OR 2.280 (0.996–5.222), P =
Table 1 Mutation spectrum for the 64 families and
0.05]. Following multivariate analysis, covarying for age, gen-
123 individuals where a mutation was identified
der, hypertension, hypercholesterolaemia and diabetes, the
association was no longer significant [OR 1.961 (0.812–
4.739), P = 0.14]. After covarying for age, there were no asso-
ciations between stroke/TIA and gender, hypertension,
hypercholesterolaemia, diabetes, ever smoking, serum choles-
terol or serum homocysteine (Table 2).
Amongst subjects with stroke/TIA, current smoking at the
time of the event was associated with earlier onset of event
(P = 0.004, Fig. 2). Following adjustment for gender, hyper-
tension, hypercholesterolaemia and diabetes, the association
with smoking remained (P = 0.01). On Cox regression there
were no associations between age of onset of stroke/TIA and
hypertension [OR 0.822 (0.470–1.439), P = 0.49], hypercho-
lesterolaemia [OR 0.787 (0.482–1.285), P = 0.34], diabetes
[OR 0.847 (0.205–3.507), P = 0.82], serum cholesterol [OR 1.015
(0.958–1.076), per 100/mmol/l, P = 0.61] or serum homo-
cysteine [OR 1.002 (0.978–1.026), per 100 Â logmmol/l, P = 0.90].
Migraine. Homocysteine level was associated with age of
All amino acid changes involved gain or loss of a cysteine (C)
onset of migraine [OR 1.024 (1.004–1.044), P = 0.02]. This
remained after controlling for age, gender and all vascular risk
Fig. 1 Quantitative lesion load and age, stratified by affected exon. The three exon 8 mutations are in the ligand-binding domain.
Table 2 Association between vascular risk factors or apoE4 allele and presence of clinical features
Odds ratios and 95% confidence intervals are given. *P = 0.02; **P = 0.03.
Fig. 3 Kaplan–Meier plot showing the difference in ages of onset
Fig. 2 Kaplan–Meier plot showing the difference in ages of onset
of migraine between individuals with high (>15 mmol/l) and
of stroke/TIA stratified according to smoking status at the time of
factors [OR 1.027 (1.006–1.048), P = 0.01]. A Kaplan–Meier
covarying for age, there was also a relationship with male
plot for migraine age of onset, comparing patients with hyper-
gender [OR 3.163 (1.130–8.854), P = 0.03]. There were no
homocysteinaemia with normal homocysteine levels, is shown
associations with hypertension, hypercholesterolaemia, dia-
in Fig. 3. Amongst the 68 subjects in whom homocysteine
betes, ever smoking, cholesterol or homocysteine (Table 2).
levels were performed, age adjusted levels were higher in
Disability. Rankin score was associated with age at disability
migraineurs compared with non-migraineurs [mean (SD)
assessment [OR 1.092 (1.044–1.143), P = 0.0001]. After cov-
homocysteine 12.8 (5.6) and 9.8 (3.4) mmol/l respectively,
arying for age, Rankin dependency was not associated with
P = 0.02 (t test on log-transformed data)].
gender, hypertension, hypercholesterolaemia, ever smoking,
A history of migraine was not associated with age [OR
serum cholesterol or homocysteine (Table 2).
1.001 (0.970–1.032), P = 0.98]. After covarying for age, ahistory of migraine was associated with homocysteinelevel [OR 1.052 (1.008–1.099) per 100 Â log mmol/l, P =
0.02]. After covarying for age, gender and all vascular risk
Quantitative MRI lesion load positively correlated with age
factors, the association was no longer significant [OR 1.047
[B regression coefficient 0.049 (0.035–0.062), P < 0.00001].
(0.997–1.099), P = 0.066]. A history of migraine was not asso-
After covarying for age, lesion load was not associated with
ciated with gender, hypertension, hypercholesterolaemia,
gender, hypertension, hypercholesterolaemia, diabetes, ever
diabetes, ever smoking or serum cholesterol (Table 2).
smoking, serum cholesterol or homocysteine (Table 3).
Scheltens score positively correlated with age [B regression
Dementia. A diagnosis of dementia was positively associated
coefficient 0.916 (0.709–1.123), P < 0.00001]. After covarying
with age [OR 1.082 (1.035–1.130), P = 0.0004]. After
for age, there was no association between Scheltens score and
Table 3 Relationships of vascular risk factors and apoE4 allele with MRI lesion load,measured quantitatively and using the semiquantitative Scheltens scale
Serum homocysteine per 100 Â log mmmol/l
B regression coefﬁcients and 95% conﬁdence intervals are shown. No associations were signiﬁcant.
gender, hypertension, hypercholesterolaemia, ever smoking,
mutational hotspot EGF-repeats 2–5 retained the ability to bind
serum cholesterol or homocysteine (Table 3).
Jagged 1 and were associated with apparently normal levels ofsignalling activity. In our study there were only three patientsfrom one family with a mutation in this domain. There was a
trend to increased age-adjusted lesion volume, measured both
quantitatively and with the Scheltens scale, but the numbers are
Amongst the 117 individuals in whom successful genotyping
too small for firm conclusions. However, such ligand-domain
was performed, the apoE genotype frequencies were: e2/e3
mutations account for only a small minority of CADASIL
7.7%, e2/e4 1.7%, e3/e3 76.1%, e3/e4 13.7%, e4/e4 0.9%.
mutations and could not account for most of the phenotypic
After covarying for age, there was no association between
the presence of at least one e4 allele and dementia, stroke/
Conventional cardiovascular risk factors, such as hyperten-
TIA or Rankin dependency (Table 2). There was no association
sion and smoking, which are associated with an increased risk
between possession of an e4 allele and quantitative MRI lesion
of sporadic small vessel disease, could potentially exacerbate
load or Scheltens score (Table 3), and mean MRI scores did not
the damage to the small cerebral vessels caused by the notch 3
significantly differ for subjects with (n = 17) or without (n = 85)
mutation. Such a gene–environment interaction could manifest
an e4 allele (lesion load, 6.14 versus 7.25%, P = 0.163; Schel-
itself as an increased tendency to acute ischaemia (lacunar
tens score, 52 versus 54, P = 0.370).
stroke) and/or more extensive chronic white matter damageseen as leucoaraiosis. Therefore, we examined associationsbetween risk factors and both clinical phenotype and age of
onset of stroke, and the extent of leucoaraiosis on MRI. We
Our results, from a large prospectively recruited series of
used two estimates of MRI lesion volume. First, we used the
CADASIL patients from a single country, found no relation-
Scheltens score, a semiquantitative scale specifically designed
ship between the mutation site and either clinical phenotype or
for evaluation of the extent of white matter ischaemic changes
the extent of white matter damage on MRI. Furthermore, there
(Scheltens et al., 1993). Secondly, we measured lesion volume
was no evidence of clustering of particular phenotypes within
from MRI scans using an image analysis programme.
families. This is consistent with previous smaller studies
We found no association between conventional risk factors,
(Dichgans et al., 1998) and suggests that the marked pheno-
including hypertension, cholesterol and smoking, and either
typic variability seen in the disease is likely to be due to addi-
MRI measure of the extent of leucoaraiosis. In contrast, there
tional modulating factors. These could include both epistatic
was an association between current smoking and age of onset of
interactions with other genes and interactions with environ-
lacunar stroke, current smokers suffering stroke at an earlier
mental risk factors. It has recently been suggested, by study of a
age. This suggests that smoking may predispose to episodes of
single family, that mutations in the recombination signal bind-
acute ischaemia, resulting in occlusion of a perforating artery.
ing protein J Kappa, ligand-binding domain might be asso-
Potential mechanisms could include induction of a prothrom-
ciated with more severe disease (Arboleda-Velasquez et al.,
botic state (Hung et al., 1995; Hioki et al., 2001) or impaired
2002). These mutations in EGFR10 and 11 are predicted to be
vasomotor function, which has been demonstrated following
required for ligand binding by homology to the Drosophila
single cigarette smoking in both animals and man (Silvestrini
melanogaster notch receptor, and functional studies have
et al., 1996; Iida et al., 1998). No other risk factors were
reported that such a mutation (C428S) exhibited a significant
associated with the presence or age of onset of stroke.
reduction in Jagged1-induced transcriptional activity of a RBP/
Despite the consistent finding that homocysteine levels
JK-responsive luciferase reporter, relative to wild-type notch
are elevated in patients with sporadic stroke (Hankey and
3, via loss of jagged binding activity (Joutel et al., 2004) In
Eikelboom, 2001) and small vessel disease in particular
contrast, other mutations (e.g. R90C and C212S) located in the
(Bertsch et al., 2001; Hassan et al., 2004), we found no
association between homocysteine level and the presence or
evidence that conventional cardiovascular risk factors may
age of onset of stroke, or the extent of MRI lesion load. In
play a modest role in modulating the phenotype in patients
contrast, there was a highly significant association between
with CADASIL. These results need confirming in prospective
elevated levels and migraine. As in other CADASIL cohorts,
CADASIL populations. However, they may have important
the great majority of cases of migraine were migraine with
implications for the management of patients with the disease.
aura. There are a number of potential mechanisms by which
The finding emphasizes the importance of smoking cessation.
elevated homocysteine could predispose to this symptom. It
Homocysteine levels can be lowered with folic acid and B
could act by exacerbating the vascular injury which presum-
vitamins (van Guldener and Stehouwer, 2001). Whether this
ably leads to both migraine and stroke. However, the lack of
will result in a reduction in the frequency of migraine needs to
association with stroke or extent of ischaemic damage would
be determined in therapeutic trials.
argue against this. Alternatively, there is evidence that homo-
Although this is one of the largest series of CADASIL
cysteine increases susceptibility to oxidative injury and exci-
patients published to date, our findings need replicating in
totoxicity, either by activation of the N-methyl-D-aspartate
larger populations. Larger studies will require international
(NMDA) subtype of glutamate receptors (Lipton et al.,
collaboration, but similarly sized CADASIL cohorts have
1997), or through DNA damage, p53 activation and subsequent
now been identified in a number of countries. Such studies
mitochondrial dysfunction (Kruman et al., 2000). All of these
will provide further understanding of the reasons for the
mechanisms have been suggested as potential mediators in
marked phenotypic heterogeneity found in CADASIL, and
migraine (Tepper et al., 2001). There is some evidence for
may also provide more fundamental information about the
an association between migraine and homocysteine in the gen-
molecular and other processes resulting in brain damage in
eral population. Higher frequencies of the C677T polymorph-
response to chronic white matter ischaemia.
(MTHFR) gene, which is associated with higher homocysteinelevels, have been found in patients with migraine compared
with controls, and particularly in patients with aura (Kowa
We wish to thank Dr Roswell Martin for help with clinical data
et al., 2000; Kara et al., 2003). Serum homocysteine levels
collection and genotyping, and Dr Yabin Dong and Kelly
were not, however, measured in these studies, and a recent
Gormley for assistance with genotyping. CADASIL case iden-
study failed to find elevated homocysteine levels in migrai-
tification was assisted by the British Neurological Surveillance
neurs compared with controls. (Hering-Hanit et al., 2001)
Unit. This work was supported by the Harrison Lectureship
The e4 allele of the apoE genotype has been found to modu-
from the Atkinson Morley Neuroscience Research Foundation.
late the extent of brain injury damage in a variety of vascularand non-vascular brain injuries, including Alzheimer’s disease(Strittmatter
(Niskakangas et al., 2001) and amyloid angiopathy (Greenberg
Andersen K, Launer LJ, Dewey ME, Letenneur L, Ott A, Copeland JR, et al.
Gender differences in the incidence of AD and vascular dementia: The
et al., 1995). We have hypothesized that it might also result in
EURODEM Studies. EURODEM Incidence Research Group. Neurology
more extensive brain injury in response to chronic ischaemia in
CADASIL patients. However, no association was found
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Health Education Kent, Surrey and Sussex Skills Development Strategy Primary and Community Care Workforce Development Programme Contents 1. Introduction and purpose 2. Key objectives of this programme 3. Education, training and workforce needs 4. Workforce profile 5. Education and Training needs identified to date in Kent Surrey and Sussex 6. Primary and Community Care Workforc
C'est la grippe finaaaaaale ! C'est la grippe finaaaaaale ! Le Citoyen Date de mise en ligne : vendredi 1er mai 2009 C'est la grippe finaaaaaale ! Après la grippe aviaire, une nouvelle forme de grippe interplanétaire apparaît. spontanément, cela va de soi ! Si l'on en croit les médias, il s'agirait d'un virus inconnu. Foulant au pied toutes les théories scientifiques, notamment