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Sc-7149 (page 1)

BACKGROUND
RECOMMENDED SECONDARY REAGENTS
MAP (mitogen-activated protein) kinases play a significant role in many bio- To ensure optimal results, the following support (secondary) reagents are logical processes, including cell adhesion and spreading, cell differentiation recommended: 1) Western Blotting: use goat anti-rabbit IgG-HRP: sc-2004 and apoptosis. p38α, p38β and p38γ, also known as MAPK14, MAPK11 and (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible goat anti- MAPK14, respectively, each contain one protein kinase domain and belong rabbit IgG-HRP: sc-2030 (dilution range: 1:2000-1:5000), Cruz Marker™ to the MAP kinase family. Expressed in different areas throughout the body Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: with common expression patterns in heart, p38 proteins use magnesium as sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunopreci- a cofactor to catalyze the ATP-dependent phosphorylation of target proteins.
pitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/ 2.0 ml). Via their catalytic activity, p38α, p38β and p38γ are involved in a variety of 3) Immunofluorescence: use goat anti-rabbit IgG-FITC: sc-2012 (dilution range: events throughout the cell, including signal transduction pathways, cytokine 1:100-1:400) or goat anti-rabbit IgG-TR: sc-2780 (dilution range: 1:100-1:400) production and cell proliferation and differentiation. The p38 proteins are with UltraCruz™ Mounting Medium: sc-24941. 4) Immunohistochemistry: use subject to phosphoryation on Thr and Tyr residues, an event which is thought ImmunoCruz™: sc-2051 or ABC: sc-2018 rabbit IgG Staining Systems.
to activate the phosphorylated protein.
REFERENCES
1. Han, J., et al. 1993. Endotoxin induces rapid protein tyrosine phosphoryla- tion in 70Z/3 cells expressing CD14. J. Biol. Chem. 268: 25009-25014.
2. Brewster, J.L., et al. 1993. An osmosensing signal transduction pathway 3. Nishida, E., et al. 1993. The MAP kinase cascade is essential for diverse signal transduction pathways. Trends Biochem. Sci. 18: 128-131.
p38α/β (H-147): sc-7149. Western blot analysis of p38α/β (H-147): sc-7149. Immunoperoxidase staining p38α expression in Jurkat (A) and KNRK (B) whole cell
of formalin-fixed, paraffin-embedded pancreas tumor showing cytoplasmic and nuclear staining.
p38α/β (H-147) is a rabbit polyclonal antibody raised against amino acids213-360 mapping at the C-terminus of p38α of human origin.
SELECT PRODUCT CITATIONS
1. Chen, G., et al. 2000. The p38 pathway provides negative feedback for Ras proliferative signaling. J. Biol. Chem. 275: 38973-38980.
Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azideand 0.1% gelatin.
2. Schultheiss, U., et al. 2001. TRAF6 is a critical mediator of signal trans- duction by the viral oncogene latent membrane protein 1. EMBO J. 20: Available as agarose (sc-7149 AC) conjugate for immunoprecipitation, 500 µg/ 3. Kumar, N. and Dey, C.S. 2002. Gliclazide increases insulin receptor tyro- APPLICATIONS
sine phosphorylation but not p38 phosphorylation in insulin-resistantskeletal muscle cells. J. Exp. Biol. 205: 3739-3746.
p38α/β (H-147) is recommended for detection of p38α and p38β of mouse,rat and human origin by Western Blotting (starting dilution 1:200, dilution 4. Kumar, N. and Dey, C.S. 2002. Metformin enhances insulin signalling in range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total insulin-dependent and -independent pathways in insulin resistant muscle protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilu- cells. Br. J. Pharmacol. 137: 329-336.
tion range 1:50-1:500), immunohistochemistry (including paraffin-embedded 5. Khurana, A. and Dey, C.S. 2003. p38 MAPK interacts with Actin and sections) (starting dilution 1:50, dilution range 1:50-1:500) and solid phase modulates filament assembly during skeletal muscle differentiation.
ELISA (starting dilution 1:30, dilution range 1:30-1:3000).
6. Hsu, M.F., et al. 2004. Mechanisms of the influence of magnolol on Positive Controls: Jurkat whole cell lysate: sc-2204.
eicosanoid metabolism in neutrophils. Biochem. Pharmacol. 67: 831-840 .
7. Chen, C.J., et al. 2004. Inhibition of inducible nitric oxide synthase expression by baicalein in endotoxin/cytokine-stimulated microglia.
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required.
8. Stoecklin, G., et al. 2004. MK2-induced tristetraprolin:14-3-3 complexes prevent stress granule association and ARE-mRNA decay. EMBO J. 23: RESEARCH USE
For research use only, not for use in diagnostic procedures.
Santa Cruz Biotechnology, Inc.
1.800.457.3801 831.457.3800 fax 831.457.3801 Europe
+00800 4573 8000 49 6221 4503 0 www.scbt.com

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