BACKGROUND RECOMMENDED SECONDARY REAGENTS
MAP (mitogen-activated protein) kinases play a significant role in many bio-
To ensure optimal results, the following support (secondary) reagents are
logical processes, including cell adhesion and spreading, cell differentiation
recommended: 1) Western Blotting: use goat anti-rabbit IgG-HRP: sc-2004
and apoptosis. p38α, p38β and p38γ, also known as MAPK14, MAPK11 and
(dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible goat anti-
MAPK14, respectively, each contain one protein kinase domain and belong
rabbit IgG-HRP: sc-2030 (dilution range: 1:2000-1:5000), Cruz Marker™
to the MAP kinase family. Expressed in different areas throughout the body
Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:
with common expression patterns in heart, p38 proteins use magnesium as
sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunopreci-
a cofactor to catalyze the ATP-dependent phosphorylation of target proteins.
pitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/ 2.0 ml).
Via their catalytic activity, p38α, p38β and p38γ are involved in a variety of
3) Immunofluorescence: use goat anti-rabbit IgG-FITC: sc-2012 (dilution range:
events throughout the cell, including signal transduction pathways, cytokine
1:100-1:400) or goat anti-rabbit IgG-TR: sc-2780 (dilution range: 1:100-1:400)
production and cell proliferation and differentiation. The p38 proteins are
with UltraCruz™ Mounting Medium: sc-24941. 4) Immunohistochemistry: use
subject to phosphoryation on Thr and Tyr residues, an event which is thought
ImmunoCruz™: sc-2051 or ABC: sc-2018 rabbit IgG Staining Systems.
to activate the phosphorylated protein. REFERENCES
1. Han, J., et al. 1993. Endotoxin induces rapid protein tyrosine phosphoryla-
tion in 70Z/3 cells expressing CD14. J. Biol. Chem. 268: 25009-25014.
2. Brewster, J.L., et al. 1993. An osmosensing signal transduction pathway
3. Nishida, E., et al. 1993. The MAP kinase cascade is essential for diverse
signal transduction pathways. Trends Biochem. Sci. 18: 128-131.
p38α/β (H-147): sc-7149. Western blot analysis of
p38α/β (H-147): sc-7149. Immunoperoxidase staining
p38α expression in Jurkat (A) and KNRK (B) whole cell
of formalin-fixed, paraffin-embedded pancreas tumor
showing cytoplasmic and nuclear staining.
p38α/β (H-147) is a rabbit polyclonal antibody raised against amino acids213-360 mapping at the C-terminus of p38α of human origin. SELECT PRODUCT CITATIONS
1. Chen, G., et al. 2000. The p38 pathway provides negative feedback for
Ras proliferative signaling. J. Biol. Chem. 275: 38973-38980.
Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azideand 0.1% gelatin.
2. Schultheiss, U., et al. 2001. TRAF6 is a critical mediator of signal trans-
duction by the viral oncogene latent membrane protein 1. EMBO J. 20:
Available as agarose (sc-7149 AC) conjugate for immunoprecipitation, 500 µg/
3. Kumar, N. and Dey, C.S. 2002. Gliclazide increases insulin receptor tyro-
APPLICATIONS
sine phosphorylation but not p38 phosphorylation in insulin-resistantskeletal muscle cells. J. Exp. Biol. 205: 3739-3746.
p38α/β (H-147) is recommended for detection of p38α and p38β of mouse,rat and human origin by Western Blotting (starting dilution 1:200, dilution
4. Kumar, N. and Dey, C.S. 2002. Metformin enhances insulin signalling in
range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total
insulin-dependent and -independent pathways in insulin resistant muscle
protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilu-
cells. Br. J. Pharmacol. 137: 329-336.
tion range 1:50-1:500), immunohistochemistry (including paraffin-embedded
5. Khurana, A. and Dey, C.S. 2003. p38 MAPK interacts with Actin and
sections) (starting dilution 1:50, dilution range 1:50-1:500) and solid phase
modulates filament assembly during skeletal muscle differentiation.
ELISA (starting dilution 1:30, dilution range 1:30-1:3000).
6. Hsu, M.F., et al. 2004. Mechanisms of the influence of magnolol on
Positive Controls: Jurkat whole cell lysate: sc-2204.
eicosanoid metabolism in neutrophils. Biochem. Pharmacol. 67: 831-840 .
7. Chen, C.J., et al. 2004. Inhibition of inducible nitric oxide synthase
expression by baicalein in endotoxin/cytokine-stimulated microglia.
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.
8. Stoecklin, G., et al. 2004. MK2-induced tristetraprolin:14-3-3 complexes
prevent stress granule association and ARE-mRNA decay. EMBO J. 23:
RESEARCH USE
For research use only, not for use in diagnostic procedures. Santa Cruz Biotechnology, Inc.
1.800.457.3801 831.457.3800 fax 831.457.3801 Europe
+00800 4573 8000 49 6221 4503 0 www.scbt.com
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BACKGROUND